Beilstein J. Nanotechnol.2022,13, 873–881, doi:10.3762/bjnano.13.78
constant (Kd) is determined by enzyme-linked oligonucleotide assay (ELONA) and the aptamer specificity is evaluated by quantitative real-time polymerase chain reaction. ZIKV60 shows a high binding affinity to the ZIKV NS1protein with a Kd value of 2.28 ± 0.28 nM. The aptamer presents high specificity for
ZIKV NS1 compared to NS1 of DENV and YFV. Furthermore, graphene field-effect transistor devices functionalized with ZIKV60 exhibit an evident identification of NS1protein diluted in human serum. These results point to the applicability of biosensors based on the ZIKV60 aptamer for the differential
diagnosis of the Zika virus.
Keywords: aptamer; biosensor; graphene; NS1protein; Zika virus; Introduction
The genus Flavivirus comprises 53 virus species that are transmitted by mosquitoes, and the majority of these have the potential to infect humans. Mosquito-transmitted viruses are a significant
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Figure 1:
Representative CE electropherogram of CE-SELEX separation step. Peak A corresponds to the NS1–aptam...